Name: and pursued my Bachelor’s education simultaneously. During

Name: RahulYadavDate ofBirth: April 3rd, 1993Course: PhD(Biological Sciences)Statementof Purpose  Improving life has always been a drivingforce ever since my conception of consciousness of this world around. Manyesteemed Scientists have spent their whole life in this invaluable and highlyrespectable work. Thus, it has always been a dream to contribute my bit to this deed. But, itbecomes a daunting task when someone belongs to a small village; where makingends meet hold priority over education and research.

Despite that, anunquenchable thirst coupled with unwavering determination leads me to travelfor nearly 4 hours/day to reach my educational destination. Having come thisfar, being a first generation learner, facing challenges and conquering each,and thus paving a way ahead to my research future, finding me doing PhD wouldbe the next step in fulfilling that dream.                     The experience of studyingand interacting with the professors, in a regular university, at my UG levelwas lacking, as I was helping in shaping the future of nearly 100 economicallybackward children each year. For almost 3 years, I was actively involved in theteaching-learning process in North Delhi Municipal Corporation and pursued myBachelor’s education simultaneously.

During that teaching-learning process, Ihad realised my insatiable desire for learning, studying, and performingexperiments because I could see the enthusiasm and ecstasy in my pupils thatimpelled me to take a new course in my life and see my dream of becoming aresearch scholar metamorphosed into a beautiful reality. Therefore, afterhelping those children, it was my turn to get guidance from a good faculty andachieve that satisfaction.                     I felt a kind of exhilaration and inflatedwith optimism when I got through and made it in the merit of 14 students whowere selected for Master’s in Biotechnology, AIIMS, out of approximate 10,000candidates, through all India entrance examination. Pursuing Biotechnology hasbeen a great journey. This institution has provided me one of the bestexperiences in research.

  My exposurehere, to new evolving technologies, broadened my educational horizon. Whether it’sthe procedure of using electron microscopy to see the cellular world or massspectrometry to analyse the different components of a chemical. But without astrong desire to learn, I would not have been able to imbibe what was impartedin the educational front.                    Learning process becomes moreenjoyable when you study as well as apply that knowledge in experiments. Thus, Practicalas well as theoretical knowledge is one of the many qualities which an AIIMSstudent possesses by having a strong curriculum. This course entailedtheoretical aspect encompassing almost all the major areas of Biology wherefirst- hand practical skill enrichment goes hand in hand followed by a yearlongthesis work.

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                    I have been doing a projectsince June 2017, as a part of my Master’s degree, entitled “To characterise GHRHR mutations causing Isolated GrowthHormone Deficiency” (Under the supervision of Dr. Arundhati Sharma, Dr. Jaya.S. Tyagi, and Dr. Renu Dhingra). In this project, I have to compare theexpression level of wild-type v/s mutant GHRHR cDNA.

Mutant cDNA was created byusing site-directed mutagenesis, to re-create the novel mutation that had beenfound in our lab (Laboratory of Cytogenetics and Molecular Biology, Departmentof Anatomy). In order to express the WT and mutant GHRHR cDNA, it was cut froma prokaryotic vector using Restriction Enzymes and then followed by ligationprocess in a mammalian expression vector, for its expression in a eukaryoticcell line. WT and mutant GHRHR cDNA was used for transfection, followed by cellharvesting. RNA and protein were isolated for qRT-PCR and western blot respectively.

                    A graduate program whichoffers prodigious research facility in this rapidly changing world by taking anintegrative approach of learning, highly motivating faculty whom I will alwayslook up to for motivation, and a positive learning environment for perpetualencouragement, are a few of the many reasons which have motivated me to apply atthe Florida State University. I am sure given the opportunity ofan educationally rich environment of the Florida State University, prodigious andinnovative faculties coupled with infrastructural facilities would certainlyhelp me realize a new dimension of this research world and thus, adding to myskills and experiences.                  PhD in Biological Sciences hasattracted my attention when I was looking for a graduate course at the FloridaState University. Endowed with an amazing faculty where everyone is making aremarkable contribution in their respective fields.                         That’sis why I am quite surethat the Florida State University is going to be a wonderful step towardsachieving my future goals and will help me in ticking all the boxes of myintellectual and creative skills.

Beingin the company of inspiring, innovative minds I would avail the opportunity tothe best of my capabilities and would make all possible efforts to make theuniversity proud by contributing my bit in the research front.                      Thanks for taking time and reading mystatement of purpose.